| SCHOOL | ASSOCIATION | HOMETOWN | SEED |
| East Northumberland SS Blue Dragons | COSSA | Brighton | 16 |
| St. Theresa CSS Titans | COSSA | Belleville | 1 |
| St. James CHS Lions | CWOSSAA | Guelph | 11 |
| Sacred Heart HS Crusaders | CWOSSAA | Walkerton | 9 |
| St Joseph’s CHS Jaguars | EOSSAA | Renfrew | 5 |
| Patrick Fogarty CSS Flames | GBSSA | Orillia | 14 |
| St Mary CSS Crusaders | GHAC | Hamilton | 12 |
| Holy Trinity CSS Titans | LOSSA | Courtice | 3 |
| Esc Hearst HS Nordik | NEOAA | Hearst | 15 |
| Korah Collegiate & VS Colts | NOSSA | Sault Ste. Marie | 7 |
| St Charles College Cardinals | NOSSA (host) | Sudbury | 8 |
| Fort Frances HS Muskies | NWOSSAA | Fort Frances | 10 |
| Saint Paul CHS Patriots | SOSSA | Niagara Falls | 13 |
| Belle River DHS Nobles | SWOSSAA | Belle River | 6 |
| Essex DHS Red Raiders | SWOSSAA | Essex | 2 |
| Parkside CI Stampeders | WOSSAA | St Thomas | 4 |