| SCHOOL | ASSOCIATION | HOMETOWN | SEED |
| Holy Cross CSS Hurricanes | COSSA | Peterborough | 1 |
| St Mary CSS Thunder | COSSA | Cobourg | 9 |
| Holy Trinity CSS | CWOSSA | Simcoe | 8 |
| Simcoe Composite School | CWOSSA | Simcoe | 16 |
| Napanee DSS Golden Hawks | EOSSAA | Napanee | 3 |
| Georgian Bay DSS Bears | GBSSA | Midland | 4 |
| Burlington CHS Blues | GHAC | Burlington | 14 |
| Henry Street HS Hawks | LOSSA | Whitby | 6 |
| St Mark HS Lions | NCSSAA | Manotick | 7 |
| É.S.C. Theriault Les Flammes | NEOAA/AANEO | Timmins | 12 |
| Lo-Ellen Park SS Knights | NOSSA | Sudbury | 11 |
| St Charles Cardinals | NOSSA (host) | Sudbury | 15 |
| Fort Frances HS Muskies | NWOSSAA | Fort Frances | 10 |
| Holy Cross CSS Raiders | SOSSA | St. Catharines | 2 |
| Lambton Central C&VI Lancers | SWOSSAA | Petrolia | 13 |
| St Michael CSS Warriors | WOSSAA | Stratford | 5 |