| SCHOOL | ASSOCIATION | SEED |
| Crestwood S.S. | COSSA | |
| Bishop Macdonell C.H.S. | CWOSSA | |
| Thousand Island S.S. | EOSSAA | |
| Holy Trinity C. H.S. | GBSSA | |
| Huntsville H.S. | GBSSA | |
| King’s Christian Collegiate | GHAC | |
| Courtice S.S. | LOSSA | |
| Paul-Desmarais ESC | NCSSAA | |
| Louis-Riel ESP | NCSSAA | |
| Chippewa S.S. | NOSSA | |
| St. Joseph-Scollard Hall C.S.S. | NOSSA – host | |
| Beaver Brae S.S. | NWOSSAA | |
| St. Paul C.S.S. | ROPSSAA | |
| Saint Paul H.S. Niagara Falls | SOSSA | |
| Centennial S.S. Welland | SOSSA | |
| É.S. L’Essor | SWOSSAA | |
| Monsignor Percy Johnson | TDCAA | |
| Harbord C.I. | TDSSAA | |
| Oakridge S.S. | WOSSAA | |
| St. Joan of Arc C.H.S. | YRAA |